主要用于肠道菌群研究使用。

从粪便样品中提取高纯度的基因组DNA菌等微生物、以及一些未消化食物样品的DNA。

由于粪便中存在大量抑制因子，常规的DNA纯化方式不能有效地去 除这些杂质，导致下游实验的失败，如PCR不能扩增出所需片段。该试剂盒采用了简便的硅胶柱纯化方式和独特的溶液系统，能有效去除动物粪便中各种影响下游 实验(如PCR)的抑制因子，并能高效地回收粪便中的基因组DNA。一次可处理0.05-0.2g样品，纯化的DNA可直接用PCR等实验。

The PSP Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Transportation of the stabilized DNA can be carried out in the DNA Stabilizer solution without refrigeration at ambient temperature. The purification kit is designed for DNA isolation from pathogenic microorganisms and from the host organism.

Benefits

· All-in-one product - from collection to isolation: pre-filled collection tubes with Stool DNA stabilizer

· Samples are stable at room temperature for 3 months for easy transport and storage

· Inactivation of DNases and preservation of the microorganism titre

· For In Vitro Diagnostic Use (CE-IVD)*

Product characteristics

Workflow

For the DNA extraction process a small aliquot of the total volume is used, and the residual sample can be stored in the DNA stabilization buffer at -20°C for further extractions. The PSP Spin Stool DNA Plus Kit uses a precleaning step, optimized buffer and essential washing conditions to remove potent inhibitors very efficiently. The purified DNA is ready to use for subsequent downstream applications:

• PCR applications
• hybridization
• genetic typing
• pathogen typing
• mutation analysis
• sequencing
• ancestry research
• feed and food determination


*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Ordering information

1. High DNA yields from stool samples

Total DNA was isolated using the PSP Spin Stool DNA Plus Kit and a competitor kit Q from 20 different stool samples according to the standard protocols. The total DNA concentration was measured on a NanoDrop (figure A) and the respective microorganism DNA content (from Bifidobacterium sp., Escherichia coli, Faecalibacterium prausnitzii, Lactobacillus sp.) was analyzed and measured via quantitative real-time PCR (figure B).

Data kindly provided by S.C. Bischoff, University Hohenheim, Stuttgart, Germany
 

2. Detection of Helicobacter pylori in human stool samples

Amplification of different target sequences of extracted bacterial DNA (H. pylori) using the PSP Spin Stool DNA Plus Kit from human stool samples stored at room temperature (without refrigeration) for 3 days with Stool Stabilizer in a Stool Collection Tube.

3. Detection of Mycobacterium avium ssp. paratuberculosis in stool samples from cattle



DNA was isolated from clinical and spiked stool samples from cattle using the PSP Spin Stool DNA Plus Kit. The isolated DNA was used for PCR amplification of the M. avium ssp. paratuberculosis target sequence ISMav2 (using the Bactotype Amplification Kit Mycobacterium paratuberculosis) with a sensitivitiy of 102 cells/g stool (6-fold repeat).

Microbiome brochure.pdf